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FAQ 1:How can I use it to analysis protein
gels and western blotting membranes,etc?
You should choose Trans-White while detecting
protein gels. When samples are opaque(such as western blotting
membrane), the White reflection light will be chose to illuminate
the top surface of the sample and reflected light enables the camera
to detect the gel.
FAQ 2: How to increase the ratio of signal to
noise ?
The longer the exposure time used to
capture an image, the more background noise builds up in the system.
This noise will often be visible on the captured image. Cameras used
for weak signals applications should be cooled. Cooling drastically
reduces inherent background noise. Some types of The Clinx GenoSens
gel documentation and analysis system are equipped with cooled CCD.
The best results are therefore obtained by having the lowest signal
to noise ratio possible.
FAQ 3:There are a number of cameras with
different specialist applications. Which type should be
used for a particular application?
When you are looking for an imaging
system you should consider all the applications you are using.
Protein gels, DNA & RNA gels, colony counting, spot blots, ELISA
plates and other images that require short exposure times, can all
be done using a standard CCD camera system.
For chemiluminescence, spot blots or antibody
assays which require much longer exposure times, a cooled CCD camera
system should be selected.Please bear in mind that at a later date
your research interests may change, resulting in a requirement to
upgrade your system. Always ensure that you can upgrade your system
with minimal cost.
FAQ 4:My images are very dark. How can I
improve them?
Firstly, check that the aperture on your lens
is fully open. In addition, all Clinx GenoSens CCD cameras have the
ability to integrate (extend the exposure time), thus improving the
brightness of the image. Using the integration control, increase the
camera exposure - this will make the image brighter. This is
especially relevant to faint bands on DNA gels. To get the correct
aperture/integration simply and quickly whilst avoiding over
exposure, use the Auto-Exposure function. The software then captures
the correct integration time for the set aperture as well as
assessing the saturation of the image during the process.
FAQ 5:What is saturation and how do I avoid
it?
Saturation occurs when an image is over
exposed. This means that the part or all of the image exceeds the
dynamic range of the camera. Once parts of an image are saturated
they are unsuitable for quantification as it is impossible to
measure genuine differences in intensity. To avoid these, you may
manually decrease the exposure time until the saturation disappear.
Another method is to use the Auto-Exposure function. The software
increases the exposure time until saturation is reached on part of
the image. It then takes off one frame (1/30 of a second) from this
time and captures, thus giving the brightest image possible without
saturation.
FAQ 6:Clinx GenoSens system has a motorized
zoom lens. What is the significance of this?
Clinx GenoSens selected the motorized zoom
lens so that the user would not need to touch the camera lens
assembly. Using a manual zoom lens means that the user can easily
contaminate the camera/lens with some very nasty chemicals. Some
chemicals crystallize upon drying and potentially prevent free
movement of parts. Having a motorized lens prevents this from
happening.
Clinx GenoSens selected the motorized zoom
lens so that the user would not need to touch the camera lens
assembly. Using a manual zoom lens means that the user can easily
contaminate the camera/lens with some very nasty chemicals.
Especially carcinogenic EB, some other chemicals crystallize upon
drying and potentially prevent free movement of parts. Having a
motorized lens prevents this from happening.
FAQ 7:I do chemiluminescence work. I cannot
see my samples by eye so I need long exposures. How long an exposure
can I set and how do I cope with the noise build-up on the image?
Most chemiluminescent exposure times will
depend on the strength of the secondary antibody and the substrate
used. Some chemiluminescent substrates work more effectively with
CCD cameras than others, those that are poor may be improved by
adjusting the secondary antibody. Exposure times vary from around 30
seconds to 8 -10 minutes. On average you should be looking at 1-2
minutes. Some types of Clinx GenoSens gel documentation and analysis
system are equipped with cooled CCD camera, which results in a
reduction of noise to almost undetectable levels.
FAQ 8:Can I use my images for publication?
Yes, the images can be used for all the
leading journals. Images in TIFF file format are usually used.
FAQ 9:Which types of printers can I use to
print my images?
Any printer can be used so long as it
has Windows drivers. The most common are thermal, dye sublimation,
inkjet or laser printers.
FAQ 10:Can the software analyze color images
taken from scanners?
The software can analyze images
up to 24 bit color.
FAQ 11:Which operating systems can I use with
my Clinx GenoSens systems?
Clinx GenoSens systems and their PC Windows
operating system requirements: Windows 2000/me/NT/XP,etc.
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